研究業績  

タイトルをクリックするとPubMed(一部はCiNii)にリンクしています。

New

2 May, 2018

Geroscience From Cell-body Dynamics and Proteostasis Cooperation Supported by αB-crystallin and Human will ~ A Proposal of “Body-Mind Integrative Science”.In: Asea A., Kaur P. (eds) Regulation of Heat Shock Protein Responses. Heat Shock Proteins, vol 13. Springer, Cham

YorikoAtomi*,MihoShimizu, EriOhto-Fujita, AyaAtomi, SaayaHayasaki, YoshikazuHigashi, TomoakiAtomi

===============================================

Gait Posture. 2017 Oct;58:415-420.

The free moment is associated with torsion between the pelvis and the foot during gait.

Ohkawa T, Atomi T, Hasegawa K, Atomi Y.

doi: 10.1016/j.gaitpost.2017.09.002. Epub 2017 Sep 5. PubMed PMID: 28898801.


PLoS One.2016 Dec 15;11(12):e0168136. doi: 10.1371/journal.pone.0168136. eCollection 2016.

SmallHeatShockProteinαB-CrystallinControlsShapeandAdhesionofGliomaandMyoblastCellsin theAbsenceofStress.

Shimizu M1,Tanaka M2,Atomi Y1.

--------------------------------------------------------

Subcell Biochem.2015;72:627-59. doi: 10.1007/978-94-017-9918-8_29.

Gravitational Effects on Human Physiology.

Atomi Y.

Abstract

Physical working capacity decreases with age and also in microgravity. Regardless of age, increased physical activity can always improve the physical adaptability of the body, although the mechanisms of this adaptability are unknown. Physical exercise produces various mechanical stimuli in the body, and these stimuli may be essential for cell survival in organisms. The cytoskeleton plays an important role in maintaining cell shape and tension development, and in various molecular and/or cellular organelles involved in cellular trafficking. Both intra and extracellular stimuli send signals through the cytoskeleton to the nucleus and modulate gene expression via an intrinsic property, namely the "dynamic instability" of cytoskeletal proteins. αB-crystallin is an important chaperone for cytoskeletal proteins in muscle cells. Decreases in the levels of αB-crystallin are specifically associated with a marked decrease in muscle mass (atrophy) in a rat hindlimb suspension model that mimics muscle and bone atrophy that occurs in space and increases with passive stretch. Moreover, immunofluorescence data show complete co-localization of αB-crystallin and the tubulin/microtubule system in myoblast cells. This association was further confirmed in biochemical experiments carried out in vitro showing that αB-crystallin acts as a chaperone for heat-denatured tubulin and prevents microtubule disassembly induced by calcium. Physical activity induces the constitutive expression of αB-crystallin, which helps to maintain the homeostasis of cytoskeleton dynamics in response to gravitational forces. This relationship between chaperone expression levels and regulation of cytoskeletal dynamics observed in slow anti-gravitational muscles as well as in mammalian striated muscles, such as those in the heart, diaphragm and tongue, may have been especially essential for human evolution in particular. Elucidation of the intrinsic properties of the tubulin/microtubule and chaperone αB-crystallin protein complex systems is expected to provide valuable information for high-pressure bioscience and gravity health science.

PMID:26174402[PubMed - indexed for MEDLINE]

******************************

英文原著論文

(Methods, invited chapter) Shimizu M, Oguro-Ando A, Ohoto-Fujita E, Atomi Y.Toll-Interacting Protein Pathway: Degradation of an Ubiquitin-Binding Protein. In P. Michael Conn, editor: Methods in Enzymology, Vol. 534 (Endosome Signalling Part A,ISBN: 978-0-12-397926-1), Burlington: Academic Press, 2014, pp. 323-330.


1. Oguro A, Kubota H, Ishiura S, Shimizu M and Atomi Y. Protective role of the ubiquitin binding protein Tollip against the toxicity of polyglutamine-expansion proteins. Neurosci Lett, 503:234-239, 2011. [査読有り]

2. Ohto-Fujita E, Konno T, Shimizu M, Kazuhiko, Ishihara K, Sugitate T, Miyake J, Yoshimura K, Taniwaki K, Sakurai T, Hasebe Y, Atomi Y., Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracelluar matrix environment for human dermal fibroblasts. Cell Tissue Res. 345,177-190, 2011. [査読有り]


3. Lee S, Sakurai T, Ohsako M, Saura R, Hatta H, Atomi Y. Tissue stiffness induced by prolonged immobilization of the rat knee joint and relevance of AGEs (pentosidine). Connective Tissue Res. 51:467-477, 2010. [査読有り]

4. Yamaguchi T., Yamaguchi T, Suzuki T, Arai H, Tanabe S, Atomi Y. Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber-type from fast to slow. Am J Physiol Cell Physiol. 298:C140-8.2010. [査読有り]

5. Jee H, Sakurai T, Kawada S, Ishii N, Atomi Y. Significant roles of microtubules in mature striated muscle deduced from the correlation between tubulin and its molecular chaperone alphaB-crystallin in rat muscles. J Physiol Sci. 2009, 59:149-55.

6. Ohto-Fujita E, Fujita Y, Atomi Y. Analysis of the alphaB-crystallin domain responsible for inhibiting tubulin aggregation. Cell Stress Chaperones. 2007, 12:163-71.

7. Yamaguchi T, Arai H, Katayama N, Ishikawa T, Kikumoto K, Atomi Y. Age-related increase of insoluble, phosphorylated small heat shock proteins in human skeletal muscle. J Gerontol A Biol Sci Med Sci. 2007, 62:481-9.

8. Otawa M, Arai H, Atomi Y. Molecular aspects of adrenal regulation for circadian glucocorticoid synthesis by chronic voluntary exercise. Life Sci. 2007, 80:725-31.

9. Oguro A, Sakurai T, Fujita Y, Lee S, Kubota H, Nagata K, Atomi Y. The molecular chaperone HSP47 rapidly senses gravitational changes in myoblasts. Genes Cells. 2006, 11:1253-65.

10. Sakurai T, Fujita Y, Ohto E, Oguro A, Atomi Y. The decrease of the cytoskeleton tubulin follows the decrease of the associating molecular chaperone alphaB-crystallin in unloaded soleus muscle atrophy without stretch. FASEB J. 2005, 19:1199-201.

11. Oguro A, Sakurai T, Okuno M, Nagata K, Atomi Y. The change of HSP47, collagen specific molecular chaperone, expression in rat skeletal muscle may regulate collagen production with gravitational conditions. Biol Sci Space. 2004, 18:150-1.

12. Fujita Y, Ohto E, Katayama E, Atomi Y. alphaB-Crystallin-coated MAP microtubule resists nocodazole and calcium-induced disassembly. J Cell Sci. 2004, 117:1719-26.

13. Tanaka M, Asashima M, Atomi Y. Proliferation and differentiation of Xenopus A6 cells under hypergravity as revealed by time-lapse imaging. In Vitro Cell Dev Biol Anim. 2003, 39:71-9.

14. Totsuka Y, Nagao Y, Horii T, Yonekawa H, Imai H, Hatta H, Izaike Y, Tokunaga T, Atomi Y. Physical performance and soleus muscle fiber composition in wild-derived and laboratory inbred mouse strains. J Appl Physiol. 2003, 95:720-7.

15. Arai H, Atomi Y. Suppression of cofilin phosphorylation in insulin-stimulated ruffling membrane formation in KB cells. Cell Struct Funct. 2003, 28:41-8.

16. Kim SG, Akaike T, Sasagaw T, Atomi Y, Kurosawa H. Gene expression of type I and type III collagen by mechanical stretch in anterior cruciate ligament cells. Cell Struct Funct. 2002, 27:139-44.

17. Atomi Y, Toro K, Masuda T, Hatta H. Fiber-type-specific alphaB-crystallin distribution and its shifts with T(3) and PTU treatments in rat hindlimb muscles. J Appl Physiol. 2000, 88:1355-64.

18. Harimoto, M., Hirose, M., Hayashi, T. and Atomi, Y. Maintenance of a round cell shape and physiological responsiveness to Insulin of mature adipocytes by culturing in type I collagen gel. Connective Tissue 32, 11-15, 2000.

19. Murakami, S., Homma, K. and Atomi, Y. "Dynamic Response to Mechanical Stimulation in Myobrasts". Trans J Soc Mechanical Engineers 66, 647 A 1432-1438, 2000.

20. Nagao Y, Totsuka Y, Atomi Y, Kaneda H, Lindahl KF, Imai H, Yonekawa H. Decreased physical performance of congenic mice with mismatch between the nuclear and the mitochondrial genome. Genes Genet Syst. 1998, 73:21-7.

21. Nagao Y, Totsuka Y, Atomi Y, Kaneda H, Lindahl KF, Imai H, Yonekawa H. Decreased physical performance of congenic mice with mismatch between the nuclear and the mitochondrial genome. Genes Genet Syst. 1998, 73:21-7.

22. Arai H, Atomi Y. Chaperone activity of alpha B-crystallin suppresses tubulin aggregation through complex formation. Cell Struct Funct. 1997, 22:539-44.

23. Atomi, Y. Decreased alpha B-cryastallin in soleus muscle atrophy and role of alpha B-crystallin in muscle. Med Sport Sci 37, 171-192, 1992.

24. Atomi Y, Yamada S, Nishida T. Early changes of alpha B-crystallin mRNA in rat skeletal muscle to mechanical tension and denervation. Biochem Biophys Res Commun. 1991, 181:1323-30.

25. Atomi Y, Yamada S, Strohman R, Nonomura Y. Alpha B-crystallin in skeletal muscle: purification and localization. J Biochem. 1991, 110:812-22.

26. Hatta H, Soma R, Atomi Y. Effect of dichloroacetate on oxidative removal of lactate in mice after supramaximal exercise. Comp Biochem Physiol B. 1991, 100:561-4.

27. Atomi, Y., Yamada, S. and Hong, Yeong-Man. Dynamic expression of alpha B-crystallin in skeletal muscle--Effects of unweighting, passive stretch and denervation--. Proc Japan Acad 66B, 203-208, 1990. PDF

28. Tabata I, Atomi Y, Kanehisa H, Miyashita M. Effect of high-intensity endurance training on isokinetic muscle power. Eur J Appl Physiol Occup Physiol. 1990, 60:254-8.

29. Tabata I, Atomi Y, Mutoh Y, Miyashita M. Effect of physical training on the responses of serum adrenocorticotropic hormone during prolonged exhausting exercise. Eur J Appl Physiol Occup Physiol. 1990, 61:188-92.

30. Hatta H, Atomi Y, Yamamoto Y, Shinohara S, Yamada S. Incorporation of blood lactate and glucose into tissues in rats after short-term strenuous exercise. Int J Sports Med. 1989, 10:275-8.

31. Tabata I, Atomi Y, Miyashita M. Bi-phasic change of serum cortisol concentration in the morning during high-intensity physical training in man. Horm Metab Res. 1989, 21:218-9.

32. Hatta H, Atomi Y, Yamamoto Y, Shinohara S, Yamada S. Oxidation of lactate in rats after short-term strenuous exercise. Int J Sports Med. 1988, 9:429-32.

33. Iwaoka K, Hatta H, Atomi Y, Miyashita M. Lactate, respiratory compensation thresholds, and distance running performance in runners of both sexes. Int J Sports Med. 1988, 9:306-9.

34. Hatta H, Atomi Y, Shinohara S, Yamamoto Y, Yamada S. The effects of ovarian hormones on glucose and fatty acid oxidation during exercise in female ovariectomized rats. Horm Metab Res. 1988, 20:609-11.

35. Atomi Y, Miyashita M. Influences of weight reduction on aerobic power and body composition of middle-aged women. J Sports Med Phys Fitness. 1987, 27:501-9.

36. Atomi Y, Fukunaga T, Hatta H, Yamamoto Y. Relationship between lactate threshold during running and relative gastrocnemius area. J Appl Physiol. 1987, 63:2343-7.

37. Atomi Y, Iwaoka K, Hatta H, Miyashita M, Yamamoto Y. Daily physical activity levels in preadolescent boys related to VO2max and lactate threshold. Eur J Appl Physiol Occup Physiol. 1986, 55:156-61.

38. Atomi, Y., Kuroda, K., Asami, T. and Kawahara, T. HDL2-Cholesterol of children (10 to 12 years of age), related to VO2max, body fat and sex. Int Series Sport Sci 17, 167-172, 1986.

39. Atomi, Y.、Fukunaga, T., Yamamoto Y. and Hatta, H. Lactate threshold and VO2max of trained and untrained boys relative to muscle mass and composition. Int Series Sport Sci 17, 167-172, 1986.

40. Atomi Y, Miyashita M. Maximal oxygen uptake of obese middle-aged women related to body composition and total body potassium. J Sports Med Phys Fitness. 1984, 24:212-8.

41. Tabata I, Atomi Y, Miyashita M. Blood glucose concentration dependent ACTH and cortisol responses to prolonged exercise. Clin Physiol. 1984, 4:299-307.

42. Atomi Y, Miyashita M. Effect of training intensity in adult females. Eur J Appl Physiol Occup Physiol. 1980, 44:109-16.

43. Atomi Y, Ito K, Iwasaki H, Miyashita M. Effect of intensity and frequency of training on aerobic work capacity of young in females. J Sports Med Phys Fitness. 1978, 18:3-9.

44. Kobayashi, S., Ohtsuki, T., Atomi, Y. and Miyashita, M. Electorical and metabolic activities during dynamic muscle contraction in man. Int. Series on BiomechanicsVolume IABiomechanics V-A, ed. by P.V. Komi, 240-245, Uni. Park Press, 1976.

45. Atomi Y, Miyashita M. Effects of moderate recreational activities on the aerobic work capacity of middle-aged women. J Sports Med Phys Fitness. 1976, 16:261-6.

46. Atomi Y, Miyashita M. Maximal aerobic power of Japanese active and sedentary adult females ofdifferent ages (20 to 62 years). Med Sci Sports. 1974 6:223-5.


Atomi&Shimizu Lab. 2013